biological source
mouse blood
packaging
tube of 5 μg 91092002-DNA-5UG, pkg of vial of cells 91092002-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblastoid
products
Cytoplasmic Immunoglobulin
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Derived from a methyl nitrosourea-induced tumour in a (C57BL/6XDBA/2) F1 mouse. This line is positive for cytoplasmic Ig and negative for surface membrane Ig and TdT.
Mouse pre-B lymphocyte
Preparation Note
Maintain cultures between 2-5x100,000 cells/ml; 5% CO2; 37°C.
RPMI 1640 + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).
Other Notes
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Depositor and originator: Dr P Kincade, Oklahoma Medical Research Foundation
法规信息
低风险生物材料
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