产品名称
MDA-MB-157, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
biological source
human breast
manufacturer/tradename
ECACC
packaging
tube of 5 μg 92020422-DNA-5UG, pkg of vial of cells 92020422-1VL
growth mode
Adherent
karyotype
Range 52-69
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Human breast medulla carcinoma
Isolated from pleural effusions of a breast cancer patient. Desmosomes, microvilli and tonofilaments observed at boundaries between cells. Ethnicity: Black.
STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 11
D5S818: 12
D7S820: 10,11
THO1: 7,8
TPOX: 9,11
vWA: 15
CSF1PO: 10
D13S317: 11
D16S539: 11
D5S818: 12
D7S820: 10,11
THO1: 7,8
TPOX: 9,11
vWA: 15
Preparation Note
L15 + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e. seeding at 3-6x10,000 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 37°C. CO2 is not required.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Use of this product by commercial organizations may require a license agreement. Commercial customers will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.
This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.
This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.