HT2 Clone A5E
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 92021401, mouse blood, Lymphoblast
生物来源
mouse blood
包装
tube of 5 μg 92021401-DNA-5UG
pkg of vial of cells 92021401-1VL
生长模式
Suspension
核型
Not specified
形态学
Lymphoblast
产品
Not specified
受体
Interleukin 2 (IL-2), Interleukin 4 (IL-4)
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
应用
IL-2 assays
生化/生理作用
Derived in 1979 from BALB/c spleen cells activated by sheep erythrocytes in the presence of IL-2, the A5E clone was selected to provide a homogenous population that is highly sensitive to IL-2. This cell line expresses Thy-1 and Lyt markers in addition to high affinity IL-2 receptors. Substantial proliferation is seen in the presence of IL-4 (GM-CSF).
Mouse helper T cell
制备说明
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. When starting from a frozen ampoule, add thawed cells to a conical based centrifuge tube e.g. 15ml tube. Slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g
RPMI 1640 + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 100-200 IU/ml IL-2 + 10% Foetal Bovine Serum (FBS).
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
法规信息
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