biological source
Pig x Pig Hybridoma
packaging
tube of 5 μg 92040117-DNA-5UG, pkg of vial of cells 92040117-1VL
ImmunoDon
Boar spleen (d/d haplotype)
Myeloma
L231 (lymphoblastoid)
Reactivity
Anti-Swine Testis (ST) cell antigen
technique(s)
cell culture | mammalian: suitable
isotype
IgA
shipped in
dry ice
storage temp.
−196°C
Immunogen
Swine testis cell culture supernatant containing transmissible gastro-enteritis virus (strain Nouzilly 188.SG)
Application
VELICA, ELISA
Biochem/physiol Actions
Pig L231 x boar spleen (d/d haplotype)
The antibody is directed against swine testis cell antigen as determined in virus enzyme linked cell immunoassays (VELCIA). The antibody does not cross-react with mouse 3T3 cells.
Preparation Note
Maintain cultures between 2-10x100,000 cells/ml; 5% CO2; 37°C.
RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 0.05mM 2-mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS)
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.