生物来源
hamster ovary
包装
tube of 5 μg 92052128-DNA-5UG
pkg of vial of cells 92052128-1VL
生长模式
Adherent
核型
Not specified
形态学
Epithelial
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
相关类别
应用
Radiation sensitivity and mutation studies.
生化/生理作用
Derived from the parent line CHO-K1 and is hypersensitive to ionising radiation but not to UV irradiation. Cells may revert to wild-type after 1-2 weeks in culture. They should be checked for sensitivity and recloned when necessary.
Hamster Chinese ovary
制备说明
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
其他说明
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