biological source
human brain
description
Human Caucasian glioblastoma
packaging
tube of 5 μg 92090213-DNA-5UG, pkg of vial of cells 92090213-1VL
growth mode
Adherent
karyotype
Hyperpentaploid, modal no. 128-132
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
T98G has been used to study the effects of the lysosomal destabilizing drug Siramesine on glioblastoma. It has also been used to study the efficiency of empty spiracles homeobox 2 (EMX2) in suppressing glioblastoma.
Studies on the mechanism for cessation of proliferation in quiescent cells and cells synchronised in G1 phase.
Biochem/physiol Actions
Human Caucasian glioblastoma
Derived from glioblastoma multiform tumour from a 61-year-old Caucasian male. Indefinite lifespan and anchorage independent but can enter viable G1 arrested state when deprived of serum.
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 13
D16S539: 13
D5S818: 10,12
D7S820: 9,10
THO1: 7,9.3
TPOX: 8
vWA: 17,20
CSF1PO: 10,12
D13S317: 13
D16S539: 13
D5S818: 10,12
D7S820: 9,10
THO1: 7,9.3
TPOX: 8
vWA: 17,20
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1% Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Cells may become anchorage-independent if deprived of serum.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.