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T98G Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 92090213, human brain, Not specified

别名:

T 98 G Cells, T-98G Cells, T98-G Cells

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UNSPSC代码:
41106514

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生物来源

human brain

描述

Human Caucasian glioblastoma

包装

tube of 5 μg 92090213-DNA-5UG
pkg of vial of cells 92090213-1VL

生长模式

Adherent

核型

Hyperpentaploid, modal no. 128-132

形态学

Not specified

产品

Not specified

受体

Not specified

技术

cell culture | mammalian: suitable

相关疾病

cancer

运输

dry ice

储存温度

−196°C

相关类别

应用

Studies on the mechanism for cessation of proliferation in quiescent cells and cells synchronised in G1 phase.
T98G has been used to study the effects of the lysosomal destabilizing drug Siramesine on glioblastoma. It has also been used to study the efficiency of empty spiracles homeobox 2 (EMX2) in suppressing glioblastoma.

生化/生理作用

Derived from glioblastoma multiform tumour from a 61-year-old Caucasian male. Indefinite lifespan and anchorage independent but can enter viable G1 arrested state when deprived of serum.
Human Caucasian glioblastoma
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 13
D16S539: 13
D5S818: 10,12
D7S820: 9,10
THO1: 7,9.3
TPOX: 8
vWA: 17,20

制备说明

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1% Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Cells may become anchorage-independent if deprived of serum.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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