biological source
mouse nerve
packaging
tube of 5 μg 92090913-DNA-5UG, pkg of vial of cells 92090913-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Neuronal
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Study of neuronal differentiation, cell adhesion and expression of neuronal proteins.
Biochem/physiol Actions
Mouse neuroblastoma x Rat neurone hybrid
ND-C hybrid cells were derived after fusion of primary neonatal-rat dorsal-root-gauglion (DRG) neurons with N18Tg2, a mouse neuroblastoma (C1300) derived azaguanine resistant line. HAT sensitive hybrids were selected and cloned by limiting dilution. The cells express rat Thy 1.1 marker and the sensory neuropeptide substance P. The cell line has been mycoplasma eradicated at ECACC.
Preparation Note
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3 x 10,000 cells/cm2; aspirate or gently tap cells loose, use of trypsin not necessary; 5% CO2; 37°C.
Other Notes
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法规信息
低风险生物材料
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