生物来源
mouse nerve
包装
tube of 5 μg 92090913-DNA-5UG
pkg of vial of cells 92090913-1VL
生长模式
Adherent
核型
Not specified
形态学
Neuronal
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
应用
Study of neuronal differentiation, cell adhesion and expression of neuronal proteins.
生化/生理作用
Mouse neuroblastoma x Rat neurone hybrid
ND-C hybrid cells were derived after fusion of primary neonatal-rat dorsal-root-gauglion (DRG) neurons with N18Tg2, a mouse neuroblastoma (C1300) derived azaguanine resistant line. HAT sensitive hybrids were selected and cloned by limiting dilution. The cells express rat Thy 1.1 marker and the sensory neuropeptide substance P. The cell line has been mycoplasma eradicated at ECACC.
制备说明
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3 x 10,000 cells/cm2; aspirate or gently tap cells loose, use of trypsin not necessary; 5% CO2; 37°C.
其他说明
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