biological source
Mouse x Mouse Hybridoma
packaging
tube of 5 μg 92100624-DNA-5UG, pkg of vial of cells 92100624-1VL
ImmunoDon
BALB/c mouse spleen
Myeloma
Sp2/0
Reactivity
Anti-Leu-5
technique(s)
cell culture | mammalian: suitable
isotype
IgM
shipped in
dry ice
storage temp.
−196°C
Immunogen
Human lymphocytes (HLA phenotype A2, A28, B27, Bw4, Cw2)
Biochem/physiol Actions
Mouse Sp2/0 x Mouse BALB/c spleen
The antibody is cytotoxic for Leu-5 positive cells. The cells require NaP, oxaloacetic acid and bovine insulin in the growth medium.
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
RPMI 1640 + 2mM glutamine + 1% Non Essential Amino Acids (NEAA) + 0.05mM 2-mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS)
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.
法规信息
动物来源培养基
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