biological source
Mouse x Mouse Hybridoma
packaging
tube of 5 μg 92100624-DNA-5UG, pkg of vial of cells 92100624-1VL
ImmunoDon
BALB/c mouse spleen
Myeloma
Sp2/0
Reactivity
Anti-Leu-5
technique(s)
cell culture | mammalian: suitable
isotype
IgM
shipped in
dry ice
storage temp.
−196°C
Immunogen
Human lymphocytes (HLA phenotype A2, A28, B27, Bw4, Cw2)
Biochem/physiol Actions
Mouse Sp2/0 x Mouse BALB/c spleen
The antibody is cytotoxic for Leu-5 positive cells. The cells require NaP, oxaloacetic acid and bovine insulin in the growth medium.
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
RPMI 1640 + 2mM glutamine + 1% Non Essential Amino Acids (NEAA) + 0.05mM 2-mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS)
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
法规信息
低风险生物材料
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