CHH-1

Aquatic viral studies

Established from normal heart tissue from a juvenile Onchorhynchus keta. Cells are susceptible to a wide range of fish viruses.

Fish Chum heart

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin/EDTA; 5% CO₂; 24°C. Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 to 1:4 and culture for at least one week with 1-2 medi

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.