biological source
rat muscle
packaging
tube of 5 μg 92121114-DNA-5UG, pkg of vial of cells 92121114-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
A subclone of L6, a rat thigh muscle cell line. On reaching confluence the cells will fuse to form myotubes and twitching movement will be seen. Once cultures have fused they cannot be recloned.
Rat skeletal muscle myoblast
Preparation Note
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split semi-confluent cultures to a seeding density of 1.5-2x1,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Cells fuse on reaching confluence, producing myotubes. To prevent fusion maintain the cultures sub-confluent and reclone every 6-8 weeks.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
法规信息
低风险生物材料
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