biological source
hamster lung
packaging
tube of 5 μg 93010723-DNA-5UG, pkg of vial of cells 93010723-1VL
growth mode
Adherent
karyotype
2n = 22
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Mutagenesis and mammalian cell genetic studies
Biochem/physiol Actions
Derived from the lung tissue of a male Chinese hamster. The cells exhibit a high plating efficiency and a doubling time of 12-14 hours. Originally used in the discovery of X-ray induced damage and recovery in mammalian cells. This is a clone of cell line V79 (Sigma Catalogue number. 86041102).
Hamster Chinese lung
Preparation Note
DMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:6 to 1:14 i.e. seeding at 3x10,000-1x100,000 cells/cm2; 5% CO2; 37°C using 0.25% trypsin/EDTA.
Other Notes
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法规信息
低风险生物材料
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