biological source
rat nerve
packaging
tube of 5 μg 93011415-DNA-5UG, pkg of vial of cells 93011415-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Bipolar
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Schwann cell gene regulation and development
Biochem/physiol Actions
Derived from the ethylnitrosourea-induced tumour line D6 of the rat peripheral nervous system. Cells will de-differentiate after 10 passages. Read references prior to use.
Rat Schwann cell, immature
Preparation Note
DMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:5 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.