NBT-II

Maturation of epidermoid carcinoma

Derived from a male Wistar rat bladder tumour induced with N-butyl-N-(4-hydroxybutyl) nitrosamine. The histotypic differentiation normally displayed by these cells is inhibited by vitamin A and gives rise to the appearance of giant cells.

Rat Wistar bladder tumour

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 2.5x1,000,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.