biological source
human blood
packaging
tube of 5 μg 93031145-DNA-5UG, pkg of vial of cells 93031145-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblast
products
Interleukin 2 (IL-2) in response to calcium ionophores and phorbol ester
receptors
TcR, CD2, CD28, CD45 (low)
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Derived from Jurkat E6-1 clone (Sigma Catalogue number. 88042803) after gamma irradiation and complement-mediated lysis with anti CD45. Low expression of CD45 (5-8% compared to parent line) results in defective signal transduction through TcR and the accessory molecule, CD2. Interleukin 2 (IL-2) is produced in response to calcium ionophore and phorbol ester stimulation, but it does not produce IL-2 in response to TcR or CD2 stimulation.
Human acute T cell leukaemia
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
法规信息
常规特殊物品
低风险生物材料
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