biological source
rat brain
packaging
tube of 5 μg 93040120-DNA-5UG, pkg of vial of cells 93040120-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Not specified
products
Plasminogen activator activity
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Study of carcinogenesis, tumourigenicity studies
Biochem/physiol Actions
Malignant cells produced on passaging BE10-Intermediate (Sigma Catalogue number. 93040119) beyond passage 40. The cells possess tissue plasminogen activator activity, are tumourigenic in BDIX rats and form colonies in agar.
Rat, BDIX, foetal brain, malignant
Preparation Note
DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 1-2x100,000 cells/25cm2 using 0.25% trypsin/EDTA; 10-15% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
法规信息
低风险生物材料
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