生物来源
rat brain
包装
tube of 5 μg 93040125-DNA-5UG
pkg of vial of cells 93040125-1VL
生长模式
Adherent
核型
Not specified
形态学
Not specified
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
应用
Study of carcinogenesis
生化/生理作用
Clone of the line BE10-Intermediate (Sigma Catalogue number. 93040119). The cells have elevated levels of tissue plasminogen activity but do not grow in agar or in BDIX rats. The cells should not be used beyond passage 24 as they start to form tumours.
Rat, BDIX, brain, pre-malignant
制备说明
DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 1-2x100,000 cells/25cm2 using 0.25% trypsin/EDTA; 10-15% CO2; 37°C.
其他说明
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法规信息
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