生物来源
mouse blood
包装
tube of 5 μg 93042610-DNA-5UG
pkg of vial of cells 93042610-1VL
生长模式
Suspension
核型
Not specified
形态学
Lymphoblast
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
相关类别
细胞系来源
Mouse C57b1/6 T cell
细胞系描述
CTLL is a subclone of T cells derived from a C57bl/6 mouse. The cells require IL-2 for growth and are used to assay for its presence in conditioned media.
应用
CTLL-2 has been used in CTLL-2 cell proliferation assay to determine the presence of T-cell cytokines.
培养基
RPMI 1640 + 2mM Glutamine + 10-20 IU/ml Human Recombinant IL-2 + 10% Foetal Bovine Serum (FBS).
传代培养常规
Maintain cultures between 3-9 x 105 cells/ml; 5% CO2; 37°C. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate volume of media to give a cell concentration of 4-5 x 105 cells/ml.
其他说明
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