生物来源
human blood
包装
tube of 5 μg 93051119-DNA-5UG
pkg of vial of cells 93051119-1VL
生长模式
Suspension
核型
Not specified
形态学
Lymphoblastoid
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
应用
Tumourigenicity studies
生化/生理作用
Derived from the peripheral blood of a 43 year old Caucasian male with widespread melanoma. The cells were transformed with defective EBV (B-95-W8).
COLO 839 (Sigma Catalogue number 93051119), COLO 858 (Sigma Catalogue number 93052613) and COLO 857 (Sigma Catalogue number 93051120) were all derived from the same 43 year-old male patient with malignant melanoma (see references below). This has been confirmed by STR profiling.
COLO 839 (Sigma Catalogue number 93051119), COLO 858 (Sigma Catalogue number 93052613) and COLO 857 (Sigma Catalogue number 93051120) were all derived from the same 43 year-old male patient with malignant melanoma (see references below). This has been confirmed by STR profiling.
Human Caucasian B lymphocyte
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,13
D13S317: 11,12
D16S539: 11,13
D5S818: 11,12
D7S820: 8,10
THO1: 9.3,10
TPOX: 8,11
vWA: 17,19
CSF1PO: 11,13
D13S317: 11,12
D16S539: 11,13
D5S818: 11,12
D7S820: 8,10
THO1: 9.3,10
TPOX: 8,11
vWA: 17,19
制备说明
Maintain cultures between 2-9x100,000 cells/ml; 5% CO2; 37°C.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
其他说明
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