COLO 800
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 93051123, human skin, Fibroblast
生物来源
human skin
包装
tube of 5 μg 93051123-DNA-5UG
pkg of vial of cells 93051123-1VL
生长模式
Adherent
核型
Not specified
形态学
Fibroblast
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
应用
Tumourigenicity studies
生化/生理作用
Derived from a subcutaneous nodule of a tumour from a 14-year-old male. Cells display remarkable blebbing.COLO 800 and COLO 794 (Sigma Catalogue number 94072237) were both derived from the same 14 year old male This has been confirmed by STR profiling.
Human melanoma
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10
D13S317: 11,13
D16S539: 11
D5S818: 11
D7S820: 10,11
THO1: 6
TPOX: 11
vWA: 16,20
CSF1PO: 10
D13S317: 11,13
D16S539: 11
D5S818: 11
D7S820: 10,11
THO1: 6
TPOX: 11
vWA: 16,20
制备说明
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 35°C; 5% CO2.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
法规信息
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