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Merck
CN

COLO 800

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 93051123, human skin, Fibroblast

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关于此项目

UNSPSC Code:
41106514
Biological source:
human skin
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Fibroblast

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biological source

human skin

packaging

tube of 5 μg 93051123-DNA-5UG, pkg of vial of cells 93051123-1VL

growth mode

Adherent

karyotype

Not specified

morphology

Fibroblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

相关类别

Application

Tumourigenicity studies

Biochem/physiol Actions

Derived from a subcutaneous nodule of a tumour from a 14-year-old male. Cells display remarkable blebbing.COLO 800 and COLO 794 (Sigma Catalogue number 94072237) were both derived from the same 14 year old male This has been confirmed by STR profiling.
Human melanoma
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10
D13S317: 11,13
D16S539: 11
D5S818: 11
D7S820: 10,11
THO1: 6
TPOX: 11
vWA: 16,20

Preparation Note

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 35°C; 5% CO2.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

法规信息

低风险生物材料
常规特殊物品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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