生物来源
human lymph
包装
tube of 5 μg 93052606-DNA-5UG
pkg of vial of cells 93052606-1VL
生长模式
Adherent
核型
Not specified
形态学
Epithelial
产品
Melanin
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
应用
Tumourigenicity studies
生化/生理作用
Derived from the right axilla lymph node of a 43 year old male with melanoma.COLO 839 (Sigma Catalogue number 93051119), COLO 858 (Sigma Catalogue number 93052613) and COLO 857 (Sigma Catalogue number 93051120) were all derived from the same 43 year-old male patient with malignant melanoma. This has been confirmed by STR profiling; COLO 853 also has an identical STR profile to the above cell lines though this cell line is not mentioned in the literature.
Human malignant melanoma
STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11,12
D16S539: 13
D5S818: 11
D7S820: 8,10
THO1: 10
TPOX: 8,11
vWA: 17,19
CSF1PO: 11
D13S317: 11,12
D16S539: 13
D5S818: 11
D7S820: 8,10
THO1: 10
TPOX: 8,11
vWA: 17,19
制备说明
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
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