biological source
human skin (brain metastasis)
packaging
tube of 5 μg 93052616-DNA-5UG, pkg of vial of cells 93052616-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
metastasis
shipped in
dry ice
storage temp.
−196°C
Application
Tumourigenicity studies
Biochem/physiol Actions
Derived from a brain metastasis of a 62 year old male with melanoma. The cells bleb during culture.
Human malignant melanoma
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11
D13S317: 11,12
D16S539: 12
D5S818: 10,12
D7S820: 10,11
THO1: 6,9
TPOX: 8,11
vWA: 16,17
CSF1PO: 11
D13S317: 11,12
D16S539: 12
D5S818: 10,12
D7S820: 10,11
THO1: 6,9
TPOX: 8,11
vWA: 16,17
Preparation Note
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.