biological source
hamster ovary
packaging
tube of 5 μg 93061607-DNA-5UG, pkg of vial of cells 93061607-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
CHO-K1/SF has been used to sequence the microRNA transcriptome and to identify, annotate and profile microRNAs as targets for cellular engineering. It has also been used to determine the pertussis toxin (PT) content of purified Bordetella pertussis fimbriae.
Biochem/physiol Actions
Hamster Chinese ovary (MEM adapted)
This MEM adapted sub-line of CHO-KI is used in Pertussis Toxin research and for quality control of batch production.
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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法规信息
低风险生物材料
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