生物来源
hamster ovary
包装
tube of 5 μg 93061607-DNA-5UG
pkg of vial of cells 93061607-1VL
生长模式
Adherent
核型
Not specified
形态学
Epithelial
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
应用
CHO-K1/SF has been used to sequence the microRNA transcriptome and to identify, annotate and profile microRNAs as targets for cellular engineering. It has also been used to determine the pertussis toxin (PT) content of purified Bordetella pertussis fimbriae.
生化/生理作用
Hamster Chinese ovary (MEM adapted)
This MEM adapted sub-line of CHO-KI is used in Pertussis Toxin research and for quality control of batch production.
制备说明
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
其他说明
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法规信息
新产品
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