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Merck
CN

LR-3

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., Reactivity: Anti-Heat Stable Antigen, mouse, 93062221

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关于此项目

UNSPSC Code:
41106514
Biological source:
Rat x Mouse Hybridoma
Technique(s):
cell culture | mammalian: suitable
Myeloma:
P3X63Ag8.653
Reactivity:
Anti-Heat Stable Antigen, mouse
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biological source

Rat x Mouse Hybridoma

packaging

tube of 5 μg 93062221-DNA-5UG, pkg of vial of cells 93062221-1VL

ImmunoDon

Rat Wistar spleen cells

Myeloma

P3X63Ag8.653

Reactivity

Anti-Heat Stable Antigen, mouse

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Immunogen

Bone marrow from Mesocestoides cortii infected BALB/c mice

Application

Detection of murine B cells, mAb appear to stimulate B cell proliferation. Detection of HSA by immunopricipitation.
The isotype of the monoclonal antibody produced by this cell line is rat IgM.

Biochem/physiol Actions

LR-3 hybridoma cells were produced by immunisation of Wistar rats with bone marrow from Mesocestoides cortii infected BALB/c cells. Subsequently rat spleen cells were fused with P3X63Ag8.653 myeloma cells.
Rat Wistar spleen x Mouse P3X63Ag8.653

Preparation Note

DMEM + 2mM glutamine + 5-10% Foetal Bovine Serum (FBS), feeder layer improves growth
Maintain cultures between 1-2x100,000 cells/ml; 5% CO2; 37°C

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

法规信息

低风险生物材料
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历史批次信息供参考:

分析证书(COA)

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