biological source
Rat x Mouse Hybridoma
packaging
tube of 5 μg 93062221-DNA-5UG, pkg of vial of cells 93062221-1VL
ImmunoDon
Rat Wistar spleen cells
Myeloma
P3X63Ag8.653
Reactivity
Anti-Heat Stable Antigen, mouse
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Immunogen
Bone marrow from Mesocestoides cortii infected BALB/c mice
Application
Detection of murine B cells, mAb appear to stimulate B cell proliferation. Detection of HSA by immunopricipitation.
The isotype of the monoclonal antibody produced by this cell line is rat IgM.
Biochem/physiol Actions
LR-3 hybridoma cells were produced by immunisation of Wistar rats with bone marrow from Mesocestoides cortii infected BALB/c cells. Subsequently rat spleen cells were fused with P3X63Ag8.653 myeloma cells.
Rat Wistar spleen x Mouse P3X63Ag8.653
Preparation Note
DMEM + 2mM glutamine + 5-10% Foetal Bovine Serum (FBS), feeder layer improves growth
Maintain cultures between 1-2x100,000 cells/ml; 5% CO2; 37°C
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.