biological source
Mouse x Mouse Hybridoma
packaging
tube of 5 μg 93062301-DNA-5UG, pkg of vial of cells 93062301-1VL
ImmunoDon
BALB/c mouse spleen
Myeloma
P3-NS1/1-Ag4.1
Reactivity
Anti-Galactocerebroside, chicken
technique(s)
cell culture | mammalian: suitable
isotype
IgG3
shipped in
dry ice
storage temp.
−196°C
Immunogen
Chicken synaptosomes
Application
Immunohistology
Biochem/physiol Actions
Mouse P3-NS1/1-Ag4.1 x Mouse BALB/c spleen
The antibody is reported to be directed against galactocerebroside present on oligodendrocytes and Schwann cells. The antibody is also reported to cross-react with related molecules in rodents and fish. Originally this antibody was reported to have an IgG1 subclass but testing at ECACC has shown it to be IgG3. However, it has not been possible to validate the antibody.
Preparation Note
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.