biological source
Human x Mouse Hybridoma
packaging
tube of 5 μg 93062401-DNA-5UG, pkg of vial of cells 93062401-1VL
ImmunoDon
Human circulating B lymphocytes
Myeloma
NS0
Reactivity
Anti-neurones
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Immunogen
None
Application
The isotype of the monoclonal antibody produced by this cell line is human IgM.
Biochem/physiol Actions
Human circulating B lymphocytes transformed with EBV. MH01 was derived from cell line 154 described in the reference given. No exogenous immunogen applied, antibody production was spontaneous.
Not specified
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37 °C.
RPMI 1640 + 2 mM glutamine + 10% Fetal Bovine Serum (FBS)
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Use of this product by commercial organizations may require a license agreement. Commercial customers will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.