COLO 677

Tumourigenicity studies

COLO 677 was originally described as being derived from a tumour in the left axillary lymph node of a 39-year-old male with small lung cell carcinoma in 1989. However, DNA fingerprinting suggests cross-contamination with cell line RPMI-8226; RPMI-8226 was established from the peripheral blood of a 61-year-old male with multiple myeloma in 1966 (see DSMZ website)

Human multiple myeloma

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Will form clusters in suspension.

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.