生物来源
mouse blood
包装
tube of 5 μg 93090713-DNA-5UG
pkg of vial of cells 93090713-1VL
生长模式
Suspension
核型
Not specified
形态学
Lymphoblast
产品
Not specified
受体
Ly-17, Lyb-2, Ly-1, Ly-5 (B220)
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
生化/生理作用
Derived from a spontaneous lymphoma in an NFS.C58v-1 mouse. These pre-B cells are negative for surface Ig, Ia and ThB.
Mouse pre-B lymphoblast
制备说明
DMEM + 10% NCTC 109 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1% Sodium Pyruvate (NaP) + 1mM Oxaloacetic acid + 50μM 2-Mercaptoethanol (2ME) + 2μg/ml Transferrin + 0.2IU/ml Insulin + 10% Foetal Bovine Serum (FBS).
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Immediately after resuscitation a pre-centrifugation step to remove cryoprotectant (DMSO) is recommended i.e. pellet the cells by centrifugation at 100 x g for 5 minutes and resuspend the cell
其他说明
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