biological source
human blood
packaging
tube of 5 μg 93093001-DNA-5UG, pkg of vial of cells 93093001-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Human B lymphocyte, EBV transformed
Peripheral blood lymphocytes from a male Tunisian immortalised by EBV transformation. The cell line contains the following deletions, in the human immunoglobulin heavy chain constant region locus (IGH chromosome 14q32.3): deletion II (del EP1-A1-GP) Gm-Am genotype Gm b; -; -A2m2/Gm g; za; .A2m2. Gm allotypes are indicated in the order G3m; G1m; G2m. The absence of G1m and G2m allotypes due to the deletion of the IGHG1 and IGHG2 genes is indicated by (-). Only one allotype is defined on the gamma 2 chain and may or may not be present; (.) indicates the absence of this allotype.
Preparation Note
Maintain cultures at 5-9x100,000 cells/ml; 5% CO2; 37°C.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.