biological source
human blood
packaging
tube of 5 μg 93093002-DNA-5UG, pkg of vial of cells 93093002-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Molecular and immunological analysis of gene deletions
Biochem/physiol Actions
Human B lymphocyte, EBV transformed
Peripheral blood lymphocytes from a male Tunisian immortalised by EBV transformation. The cell line contains the following deletions in the human immunoglobulin heavy chain constant region locus: deletion I (G1-EP1-A1-GP-G2-G4) and deletion II (EP1-A1-GP). Gm-Am phenotype: Gmb:-;A2m2/Gmg;za;.A2m2. The absence of G2m allotypes due to the deletion of the IGHG2 gene.
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.