biological source
Unknown
packaging
tube of 5 μg 93100514-DNA-5UG, pkg of vial of cells 93100514-1VL
ImmunoDon
Not specified
Myeloma
Not specified
Reactivity
Anti-Leukocyte Molecules, sheep
technique(s)
cell culture | mammalian: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−196°C
Immunogen
Sheep lymphocytes
Biochem/physiol Actions
The antibody is directed against a molecule expressed on most sheep leukocytes, platelets and endothelium. Its molecular weight could not be determined but may be equivalent to sheep CD3. The molecule is down-regulated in activated and memory T cells.
Preparation Note
IDMEM + 2mM glutamine + 1% Hypoxanthine, Aminopterin, Thymidine (HAT) + 10% Foetal Bovine Serum (FBS) (may benefit from IL-6 in medium)
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.