biological source
Porcine intestine
packaging
tube of 5 μg 93100621-DNA-5UG, pkg of vial of cells 93100621-1VL
growth mode
Adherent
karyotype
Heteroploid
morphology
Fibroblast-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Potential feeder cell line for the recovery of epithelial cells isolated from the small intestine.
Biochem/physiol Actions
Boar Miniature male ileum, SV40 transformed
Fibroblast cell line derived from the ileum of an adult histocompatible miniature male boar. Transformed by SV40 plasmid pSV3-neo.
Preparation Note
DMEM + 4mM Glutamine + 0.024IU/ml Insulin + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
法规信息
低风险生物材料
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