biological source
Mouse x Mouse Hybridoma
packaging
tube of 5 μg 93102701-DNA-5UG, pkg of vial of cells 93102701-1VL
ImmunoDon
BALB/c mouse spleen
Myeloma
Sp2/O
Reactivity
Anti-Lymphocytes/endothelium, sheep
technique(s)
cell culture | mammalian: suitable
isotype
IgM
shipped in
dry ice
storage temp.
−196°C
Immunogen
Sheep lymphocytes
Biochem/physiol Actions
Mouse Sp2/O x Mouse BALB/c spleen
The antibody is directed against antigen expressed on most sheep lymphocytes and also endothelium. The antigen recognised by 537-B1-1 is down regulated after T cell activation.
Preparation Note
IDMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS) (may benefit from IL-6 in medium)
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.
法规信息
动物来源培养基
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