生物来源
fish skin
包装
tube of 5 μg 93120820-DNA-5UG
pkg of vial of cells 93120820-1VL
生长模式
Adherent
核型
2n = 96
形态学
Epithelial
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
生化/生理作用
Fish epithelioma Fathead minnow Pimephales promelas
Not specified
This cell line known as Epithelioma papulosum cyprini (EPC) was originally reported to be from Carp (Cyprinus carpio) epidermal herpes virus-induced hyperplastic lesions. More recently EPC was found to be derived from Fathead Minnow (Pimephales promelas) see Winton et al., 2010. This has been confirmed by DNA sequencing at ECACC. The cells support the growth of pancreatic necrosis virus and rhabdovirus.
制备说明
EMEM (EBSS) + 2 mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 using 0.25% trypsin/EDTA; 5% CO2; 20 °C. Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 and culture for at least one week with 1-2 media changes before shipping.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
法规信息
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