M1

Myeloid differentiation studies

Established from a spontaneous myeloid leukaemia of a SL strain mouse. Cells have been used as a model for differentiation in the myeloid macrophage pathway and can be induced to become macrophage-like by a variety of stimulating agents including dexamethasone.

Mouse myeloblast

Maintain cultures between 2-9x100,000 cells/ml; 5% CO₂; 37°C. Subculture before confluency, cells become adherent if grown at high density.

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.