生物来源
mouse skin
包装
tube of 5 μg 93120827-DNA-5UG
pkg of vial of cells 93120827-1VL
生长模式
Adherent
核型
Modal no. 83; hypertetraploid
形态学
Epithelial
产品
Melanin
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
细胞系来源
Mouse Cloudman S 91 melanoma
细胞系描述
Melanin producing cell line, derived from a Cloudman S 91 melanoma induced in a F1 (CxDBA) male mouse. The line is tumourigenic in mice and susceptible to a variety of viruses including herpes simplex, vaccinia, pseudorabies and vesicular stomatitis virus (Indiana virus).
应用
Tumourigenicity studies
培养基
Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
其他说明
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