生物来源
human lung
包装
tube of 5 μg 93120837-DNA-5UG
pkg of vial of cells 93120837-1VL
生长模式
Adherent
核型
2n = 46
形态学
Epithelial
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
细胞系来源
Human Caucasian lung squamous carcinoma
细胞系描述
Derived from the pleural effusion of a 65 year old Caucasian male with squamous cell carcinoma of the lung. HLA = A3, B7, B27, w30.
应用
Tumourigenicity studies
DNA图谱分析
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 12
D13S317: 11
D16S539: 13
D5S818: 11
D7S820: 8
THO1: 6,9.3
TPOX: 8
vWA: 14
CSF1PO: 12
D13S317: 11
D16S539: 13
D5S818: 11
D7S820: 8
THO1: 6,9.3
TPOX: 8
vWA: 14
培养基
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
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