biological source
mouse pancreas
packaging
tube of 5 μg 94022430-DNA-5UG, pkg of vial of cells 94022430-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Not specified
products
Somatostatin
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Study of exocrine pancreas carcinomas, hormone secretion studies.
Biochem/physiol Actions
Mouse pancreatic islet cell tumour
TGP 54 has been isolated from an islet tumour arising in the pancreas of a transgenic Tg (E1a-1-SV40E)Bri8 mouse. These mice carry a pseudogene construct composed of a pancreatic-specific elastase-1 promoter linked to the SV40T antigen and thereby represent an animal model for pancreatic carcinogenesis. TGP 54 cells are SV40T antigen positive and secrete low amounts of somatostatin, it is one of a series of cells obtained from pancreatic tumours deposited at ECACC.
Preparation Note
DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).
Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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法规信息
低风险生物材料
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相关内容
ECACC pancreatic cancer cell lines for various applications including drug response studies.
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