Hs 27

Human foreskin, fibroblast

Hs27 is one of a series of human foreskin fibroblast lines developed at NBL, California. The material was obtained from a normal new-born, and the cells possess the G6PD type A phenotype. Longevity studies carried out at NBL demonstrated that the cells can be propagated for 42 passages. There were no consistent aberrations in analysed cells. Ethnicity: Black.

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.