biological source
human muscle
packaging
tube of 5 μg 94042258-DNA-5UG, pkg of vial of cells 94042258-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
metastasis
shipped in
dry ice
storage temp.
−196°C
Application
Carcinogenesis studies, metastatic ability experiments.
Biochem/physiol Actions
Human chondrosarcoma
This extraskeletal myeloid chondrosarcoma has been derived from the muscle of a 77 year old female with metastases found in lymph nodes and lung.
Preparation Note
Ham′s F12/DMEM (1:1) + 10 % Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:4 i.e. seeding at 2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.