GRL101 (MIX)

Studies in the newly characterised members of a putative class of G protein - linked receptors that directly transduce signals carried by large extracellular (lipo)protein (complexes) into neural events.

Cell line containing the receptor protein GRL101. The cDNA from the CNS of the mollusc Lymnaea stagnalis encoding a putative receptor has been isolated and analysed. The first repeat of DNA displays a high sequence similarity to the extracellular binding of the low density protein receptor, which binds and internalises cholesterol containing apolipoproteins. The MRNI receptor is predominantly expressed in a small number of neurons within the CNS and to a lesser extent in the heart. Contains a mixed culture of approximately 20 transfected clones.

Hamster Chinese ovary transfected with Lymnaea receptor GRL101

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2 - 4 x 10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂, 37°C.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.