GRL101 (KC7)

Cell line containing the receptor protein GRL101. The cDNA from the CNS of the mollusc Lymnaea stagnalis encoding a putative receptor has been isolated and analysed. The first repeat of DNA displays a high sequence similarity to the extracellular binding domains of the low density protein receptor, which binds and internalises cholesterol containing apolipoproteins. The MRNI receptor is predominantly expressed in a small number of neurons within the CNS and to a lesser extent in the heart.

Hamster Chinese ovary transfected with Lymnaea receptor GRL101

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂, 37°C.

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