Study of human oesophageal cancer.

Human Asian squamous cell carcinoma

KYSE-30 was established from the oesophageal cancer of an untreated 64 year old male. The tumour sample was taken from the mucosal surface of a well differentiated squamous cell carcinoma. The cell line KYSE-30 was established with the use of tumours initially transplanted to athymic mice. The cells are reported to have a doubling time of 20.8 hrs in the exponential growth phase. A p53 mutation at the splice acceptor site of intron 6 and a 12 fold amplification of c-erb B has been reported. KYSE-30 cells express a large number of epidermal growth factor receptors, 1.2x10,000,000 sites/cell.

RPMI 1640 + Ham′s F12 (1:1) + 2mM Glutamine + 2% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:10 i.e. seeding at 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

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