biological source
human esophagus
packaging
tube of 5 μg 94072012-DNA-5UG, pkg of vial of cells 94072012-1VL
growth mode
Adherent
karyotype
Hypotriploid with 8% polyploidy
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Established from a poorly differentiated invasive oesophageal squamous cell carcinoma resected from middle intra-thoracic oesophagus of a 77-year-old Japanese man prior to treatment. This cell line has been mycoplasma eradicated at ECACC. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line. It is a known phenomenon that SSC cell lines can lose their Y chromosome.
Human oesophageal squamous cell carcinoma
Preparation Note
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Split confluent cultures 1:5 to 1:10 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Doubling time ~35 hours.
Other Notes
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法规信息
常规特殊物品
低风险生物材料
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