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主要文件

KYSE-70

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 94072012, human esophagus, Epithelial

别名:

KY70 Cells, KYSE70 Cells

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About This Item

UNSPSC代码:
41106514

生物来源

human esophagus

包装

tube of 5 μg 94072012-DNA-5UG
pkg of vial of cells 94072012-1VL

生长模式

Adherent

核型

Hypotriploid with 8% polyploidy

形态学

Epithelial

产品

Not specified

受体

Not specified

技术

cell culture | mammalian: suitable

相关疾病

cancer

运输

dry ice

储存温度

−196°C

细胞系来源

Human oesophageal squamous cell carcinoma

细胞系描述

Established from a poorly differentiated invasive oesophageal squamous cell carcinoma resected from middle intra-thoracic oesophagus of a 77-year-old Japanese man prior to treatment. This cell line has been mycoplasma eradicated at ECACC. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line. It is a known phenomenon that SSC cell lines can lose their Y chromosome.

培养基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

传代培养常规

Split confluent cultures 1:5 to 1:10 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Doubling time ~35 hours.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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