KYSE-70
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 94072012, human esophagus, Epithelial
别名:
KY70 Cells, KYSE70 Cells
生物来源
human esophagus
包装
tube of 5 μg 94072012-DNA-5UG
pkg of vial of cells 94072012-1VL
生长模式
Adherent
核型
Hypotriploid with 8% polyploidy
形态学
Epithelial
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
生化/生理作用
Established from a poorly differentiated invasive oesophageal squamous cell carcinoma resected from middle intra-thoracic oesophagus of a 77-year-old Japanese man prior to treatment. This cell line has been mycoplasma eradicated at ECACC. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line. It is a known phenomenon that SSC cell lines can lose their Y chromosome.
Human oesophageal squamous cell carcinoma
制备说明
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Split confluent cultures 1:5 to 1:10 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Doubling time ~35 hours.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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