Human lung adenocarcinoma

Established from the pleural effusion of a pulmonary adenocarcinoma in a 51 year-old Japanese female. LC-2/ad cells exhibit an epithelial appearance and a tendency to form small domes. Immunocytochemically the cells are positive for CEA and cytokeratins, including cytokeratin No.18. LC-2/ad produces at least two functionally active trypsin inhibitors together with several proteinases in vitro.

LC-2/ad has been used to study the ability of RXDX-105 as a therapy for cancers which are affected by RET (proto-oncogene tyrosine-protein kinase receptor) changes.

RPMI 1640:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Split confluent cultures 1:2-1:4 ie seeding at 2-5 x 10,000 cells/cm² using 0.25% trypsin or trypsin/ EDTA ; 5%CO₂; 37°C

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