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关于此项目
UNSPSC Code:
41106514
Biological source:
human nerve (bone marrow metastasis)
Relevant disease(s):
metastasis
Growth mode:
Adherent
Karyotype:
44XX, monosomic for 10, 11, 13, 14, 19. Both copies of chromosome 2 missing in each cell.
Morphology:
Neuronal
Manufacturer/tradename:
ECACC
biological source
human nerve (bone marrow metastasis)
manufacturer/tradename
ECACC
packaging
tube of 5 μg 94092305-DNA-5UG, pkg of vial of cells 94092305-1VL
growth mode
Adherent
karyotype
44XX, monosomic for 10, 11, 13, 14, 19. Both copies of chromosome 2 missing in each cell.
morphology
Neuronal
products
p53, moderate MDR-1 expression
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
metastasis
shipped in
dry ice
storage temp.
−196°C
Application
Tumour studies. MRD-1 resistance studies. Extremely sensitive to cis-retinoic acid.
Biochem/physiol Actions
Human neuroblastoma
STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 8,11
D16S539: 9,11
D5S818: 12
D7S820: 12,13
THO1: 6,9
TPOX: 8
vWA: 16,18
CSF1PO: 12
D13S317: 8,11
D16S539: 9,11
D5S818: 12
D7S820: 12,13
THO1: 6,9
TPOX: 8
vWA: 16,18
Spontaneously transformed cells from a female patient with neuroblastoma. The tumour was derived from a bone marrow metastasis and is tumourigenic in nude mice. Used in the study of MIBG uptake in the diagnosis and treatment of neuroblastoma. MIBG is a derivative of the antihypertensive agent guanethidine and is used as a radiopharmaceutical. SK-N-DZ is characterised by low saturable kinetics and predominant linear pattern of uptake of MIBG, with a poor ability to maintain high intracellular levels of the drug.
Preparation Note
DMEM or EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.