生物来源
rat liver
包装
tube of 5 μg 94101906-DNA-5UG
pkg of vial of cells 94101906-1VL
生长模式
Adherent
核型
Hyperdiploid, 52 Chromosomes
形态学
Epithelial
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
细胞系来源
Rat hepatoma [HGPRT-]
细胞系描述
Full name Faof1C2 is a dedifferentiated variant of Fao. These cells continue to express tyrosine aminotransferase, but the liver-specific markers are mostly absent.
应用
Gene expression analysis.
培养基
Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
传代培养常规
Split semi-confluent cultures 1:50 i.e. seeding at 2x1000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Saturation density is reached at 10x10,000,000,000 cells/10cm2 dish.
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