PG-4

PG-4 cells can be used to detect and assay type C retroviruses and are currently recommended by the FDA for the detection of replication competent retroviruses in gene therapy products and hybridomas producing reagents for human use.

Cells can be used to detect and assay type C retroviruses and are currently recommended by the FDA for the detection of replication competent retroviruses in gene therapy products and hybridomas producing reagents for human use.

Cat brain Moloney sarcoma virus-transformed

PG-4 was derived at NIH in 1980 from G355 cells by transformation with Moloney Murine sarcoma virus (M-MST) and subsequently cloned. Support the growth of simian sarcoma associated virus (SSAV); macaque virus; feline immunodeficiency virus (FeLV) A, B and C; feline RD-114; C retroviruses; murine xenotropic and munk cell focus-inducing virus (MCF).

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (heat-inactivated).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.