WEHI-279

Derived from a tumour induced in an NZC mouse by irradiation, the cell line represents an early stage of B cell development characterised by high-density membrane immunoglobulin expression (IgGM, kappa) but no detectable immunoglobulin secretion.

Mouse B cell lymphoma

DMEM + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).

Maintain cultures between 5x10,000 to 2x100,000 cells/ml; 5% CO₂: 37°C.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.