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关于此项目
UNSPSC Code:
41106514
Biological source:
Horse x Mouse/Horse Hybridoma
Isotype:
Not specified
Technique(s):
cell culture | mammalian: suitable
Myeloma:
NS0
Reactivity:
Application: Fusion partner for hybridoma production
biological source
Horse x Mouse/Horse Hybridoma
packaging
tube of 5 μg 94120825-DNA-5UG, pkg of vial of cells 94120825-1VL
ImmunoDon
Horse peripheral lymphocytes
Myeloma
NS0
Reactivity
Application: Fusion partner for hybridoma production
technique(s)
cell culture | mammalian: suitable
isotype
Not specified
shipped in
dry ice
storage temp.
−196°C
Immunogen
Influenza A equine 2 virus
Application
Fusion partner for hybridoma production
Biochem/physiol Actions
Horse peripheral blood lymphocytes x Mouse NS0
The heterohybridoma AFRC 17D1 was obtained by fusion of lymphocytes from ponies immunised with the influenza A equine 2 virus (isolateA/Equine/Newmarket/79 (H3N8)) with mouse myeloma NS0 cells. The hybridoma was made aminopterin-sensitive by selective growth in 8-azaguanine. This non-Ig-secreting cell line was used as fusion partner for the establishment of equine-mouse heterohybidomas.
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C
RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 5% Foetal Bovine Serum (FBS)
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.