biological source
Horse x Mouse/Horse Hybridoma
packaging
tube of 5 μg 94120832-DNA-5UG, pkg of vial of cells 94120832-1VL
ImmunoDon
Horse inguinal lymph node
Myeloma
AFRC 17D1
Reactivity
Anti-Haemagglutinin, influenza
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Immunogen
Influenza A equine 2 virus
Application
Haemagglutinin detection
The isotype of the monoclonal antibody produced by this cell line is horse IgG.
Biochem/physiol Actions
Horse inguinal lymph node x AFRC 17D1
The heterohybridoma AFRC 85B5 is one of 8 hybridoma cell lines obtained using lymphocytes from ponies immunised with influenza A equine 2 virus (isolateA/Equine/Newmarket/79(H3N8)). For the establishment of AFRC 85B5 horse cells from the inguinal lymph node have been fused with the non-Ig-secreting heterohybridoma AFRC 17D1 (Sigma Catalogue number. 94120825).
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C
RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 5% Foetal Bovine Serum (FBS)
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.
法规信息
动物来源培养基
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